development of a unique set of polymeric hybrid constructs (hereafter called 20Med-nanogels) which possess an unmet combination of properties essential for successful siRNA delivery in clinical applications.

20Med-nanogels are stable, well-processable, nontoxic and biodegradable nanoparticles that can be stored as powder formulation and can be easily loaded with oligonucleotides after dissolution.

Specific chemical functionalities within the 20Med-nanogel architecture promote endosomal escape and efficient release of the therapeutic payload into the cytosol after the nanoparticles are taken up by the cells, resulting in high transfection and gene silencing efficiencies.

Moreover, due to their special chemical construction, 20Med nanogels are very flexible in tuning chemical functionalization and molecular architecture, which makes them also excellently suitable for developing tailor-made carriers for targeted delivery of therapeutic peptides, proteins, and small molecules.